Coding

Part:BBa_K4129009:Design

Designed by: Christian Wistrm   Group: iGEM22_DTU-Denmark   (2022-10-02)


Oxidoreductase, oxidising furfural and furfuryl alcohol til furoic acid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 393
    Illegal PstI site found at 211
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1656
    Illegal PstI site found at 211
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1197
    Illegal BamHI site found at 46
    Illegal BamHI site found at 84
    Illegal XhoI site found at 1049
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 393
    Illegal PstI site found at 211
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 393
    Illegal PstI site found at 211
    Illegal NgoMIV site found at 820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 310
    Illegal BsaI site found at 1128
    Illegal BsaI.rc site found at 188


Design Notes

nucleotide sequence was codon optimized for Aspergillus niger using GenSmart's codon optimizer tool


Source

from Cupriavidus basilensis

References